Journal: Journal of Clinical and Experimental Hematopathology : JCEH

Article Title: Clinicopathological features of gastric Langerhans cell histiocytosis and a literature review

doi: 10.3960/jslrt.25082

Figure Lengend Snippet: The endoscopic presentation and pathological features of gastric LCH A : Endoscopic examination revealed a small polyp in the gastric body, measuring 0.5 cm in diameter. B : The tumor cells are located in the mucosal layer and show clear boundaries with the surrounding tissue (H&E, 200×). C : The tumor cells exhibit round or oval nuclei, with some cells showing nuclear grooves. Eosinophils are scattered in the background, and occasional mitotic figures are observed (H&E, 400×). D : Diffuse expression of Langerin (EnVision method, 400×). E : Diffuse expression of CD1a (EnVision method, 400×). F : Positive expression of Cyclin D1 (EnVision method, 400×). G : Positive expression of S-100 (EnVision method, 400×). H : Positive expression of Ki-67 (EnVision method, 200×).

Article Snippet: The primary antibodies used included S-100 (mouse monoclonal, clone 4C4.9, 1:150; Zhongshan Golden Bridge Biotechnoloy Co., Ltd., Beijing, China), Langerin (mouse monoclonal, clone 12D6, Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China), Cyclin D1 (mouse monoclonal, clone SA38‐08, Zhongshan Golden Bridge Co., Ltd., Beijing, China), CD68 (mouse monoclonal, clone KP-1, 1:200; Maixin Biotechnology Development Co., Ltd., Beijing, Fuzhou, China), CD1a (mouse monoclonal, clone O10, 1:100; Maixin Biotechnology Development Co., Ltd., Beijing, Fuzhou, China), CD20 (mouse monoclonal, clone L26, 1:100; Maixin Biotechnology Development Co., Ltd., Beijing, Fuzhou, China), AE1/AE3 (mouse monoclonal, clone AE1/3, 1:200; Gene Technology Co., Ltd., Shanghai, China), Ki‐67 (mouse monoclonal, clone GM027, 1:200; Gene Technology Co., Ltd., Shanghai, China), and CD3 (mouse monoclonal, clone LN10, 1:100; Gene Technology Co., Ltd., Shanghai, China).

Techniques: Expressing

Journal: Animal Bioscience

Article Title: Transcriptomic analysis identifies CXCL12 as a novel candidate gene for litter size in rabbits

doi: 10.5713/ab.24.0640

Figure Lengend Snippet: CXCL12 overexpression and knockdown in GCs. (A,B) Changes in the expression of CXCL12 after its overexpression and knockdown in GCs. (C,D) The mRNA expression level of PCNA , CCND1 , CDK2 , Bcl-2 and Bax was detected after overexpressing or knocking down CXCL12 in GCs. (E) The protein expression levels of CCND1, PCNA, Bcl-2 and Bax were detected after overexpressing and knocking down CXCL12 in GCs. H, high-litter size group; L, low-itter size group; GAPDH was selected as the internal reference gene (The t test was used for the above analyses comparing two individual samples. * p<0.05; ** p<0.01; *** p<0.001; ns, not significant). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, granulosa cells.

Article Snippet: Protein detection was achieved using the following antibodies: anti-CCND1 mouse monoclonal antibody (1:250, Proteintech), anti-PCNA rabbit polyclonal antibody (1:250, Proteintech), anti-Bcl2 rabbit polyclonal antibody (1:250, Proteintech), anti-Bax rabbit polyclonal antibody (1:250, Proteintech), anti-CITED1 rabbit polyclonal antibody (1:50, Proteintech), anti-WNT10B mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-CXCR4 mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-phospho-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam, Cambridge, UK), anti-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam), anti-phospho-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-GAPDH mouse monoclonal antibody (1:2,500, Proteintech), 1:1,000 goat anti-rabbit secondary antibody IgG (Proteintech) and 1:1,000 goat anti-mouse secondary antibody IgG (Proteintech).

Techniques: Over Expression, Knockdown, Expressing

Journal: PLOS One

Article Title: STAG2 mutations in the normal colon induce upregulation of oncogenic pathways in neighbouring wildtype cells

doi: 10.1371/journal.pone.0332499

Figure Lengend Snippet: a. Heatmap depicting the top 20 differentially expressed genes between STAG2 wildtype and mutant populations. b. Using gene set enrichment analysis (GSEA), only the “HALLMARK_TNFA_SIGNALING_VIA_NFKB” gene set was upregulated in wildtype compared to mutant (Normalised enrichment score: 1.75, False discovery rate q -value: 0.078). c. On immunofluorescence staining, we observed increased proliferation of wildtype organoids relative to STAG2 mutants when stained with anti-KI67 antibody. d. Fluorescent intensity of anti-KI67 antibody was statistically significantly upregulated in co-cultured wildtype organoids relative to STAG2 mutant organoids. e. We also observed increased tumorigenicity of wildtype organoids relative to STAG2 mutants when stained with anti-CCND1 antibody. f. Fluorescent intensity of anti-KI67 antibody was statistically significantly upregulated in co-cultured wildtype organoids relative to STAG2 mutant organoids. Scale bars are 50µm. All experiments were performed with N = 3 biological replicates.

Article Snippet: Primary antibodies used included rabbit anti-human STAG2 antibody (1:100, 19837–1-AP, Proteintech, USA), mouse anti-human KI67 antibody (1:500, 66555–6-Ig, Proteintech, USA), mouse anti-human P53 antibody (1:400, 60283–2-Ig, Proteintech, USA), mouse anti-human CCND1 antibody (1:100, 60186–1-Ig, Proteintech, USA), mouse anti-human TERT antibody (1: 100, MA5−16033, Invitrogen, USA), mouse anti-human KRAS antibody (1:250, 415700, Invitrogen, USA), and mouse anti-human TNFα antibody (1:50, MA5−23720, Invitrogen, USA).

Techniques: Mutagenesis, Immunofluorescence, Staining, Cell Culture